RNA polymerase mutation activates the production of a dormant antibiotic 3,3'-neotrehalosadiamine via an autoinduction mechanism in Bacillus subtilis.

RNA polymerase mutation activates the production of a dormant antibiotic 3, 3’-neotrehalosadiamine via autoinduction mechanism in Bacillus subtilis

Glucose uptake pathway-specific regulation of synthesis of neotrehalosadiamine, a novel autoinducer produced in Bacillus subtilis.

Neotrehalosadiamine (3,3'-diamino-3,3'-dideoxy-alpha,beta-trehalose; NTD) is an amino-sugar antibiotic produced by several Bacillus species that functions as an autoinducer by activating its own biosynthetic operon, ntdABC. We previously reported that the introduction of a certain rpoB mutation (rpoB5) into Bacillus subtilis enables the cells to overproduce NTD. B. subtilis mini-Tn10 transposan...

Isolation of an aminoglycoside hypersensitive mutant and its application in screening.

An aminoglycoside hypersensitive mutant, Kp-126, was isolated from the aminoglycoside-resistant strain, Kp-8, of Klebsiella pneumoniae through selection using sorbistin, a non-aminocyclitol-aminoglycoside antibiotic. The mutant Kp-126 was approximately 100-fold more sensitive to sorbistin than the parent strain Kp-8. The mutant also showed hypersensitivity to various aminocyclitol-aminoglycosid...

Transcription after bacteriophage SPP1 infection in Bacillus subtilis.

The role of the host polymerase in Bacillus subtilis infected with phage SPP1 was studied in vivo with regard to production of phage-specific and host-specific ribonucleic acid (RNA) and to phage yield. Evidence is presented that the subunit(s) of B. subtilis RNA polymerase which is sensitive to rifampin and streptolydigin is necessary at all times during infection for phage production. The syn...

In vitro elaboration Mutagenesis and cloning of the PA gene in Bacillus subtilis

Background: The immune antigen of Bacillus anthracis is a protein that can attach to the surface receptor of all human cells. At the surface of cancer cells, there is a receptor that activates the uPA (Urokinase plasminogen) that do not exist in normal human cells. Objectives: The aim of this study was changing the location of the attachment of the PA gene by a dir...